Effect on Mouse Liver Morphology of CeO2, TiO2 and Carbon Black Nanoparticles Translocated from Lungs or Deposited Intravenously

Exposure to nanoparticles by various routes results in size-dependent translocation of the systemic circulation and subsequent accumulation liver. The purpose this study was determine possible adverse effects liver long-lasting nanoparticle presence organ. Mice exposed a single dose (162 µg/animal equivalent 9 mg/kg body weight) TiO2, CeO2 or carbon black intratracheal instillation intravenous injection, resulting relatively low high burdens, were followed for 1, 28 180 days. Clinical appearance, feed intake, weights, hematological indices, transaminases alkaline phosphatase activities unaffected exposure. Exposure-related foreign material persisted up days after exposure, mainly sinusoids, near Kupffer cells, around blood vessels. Increased incidences histological findings exposure included: initially, prominent nuclei apparent increase binucleate hepatocytes (TiO2 black) inflammatory infiltrations (CeO2); later, cytoplasmic vacuolation, pyknosis necrosis, especially CeO2. Thus, neither nor burden affected enzymatic markers injury, but indications exposure-related necrotic changes, particularly nanoparticles, noted.